ANGSD: Analysis of next generation Sequencing Data
Latest tar.gz version is (0.938/0.939 on github), see Change_log for changes, and download it here.
Mismatch
Angsd can output mismatch from the reference given:
- - Distance from be beginning of the read (posi)
- - Distance from the end of the read (isop)
- - Strand
- - Qscore
Run like:
./angsd -i my.cram -ref hg19.fa -doMisMatch
Output looks like:
posi isop qs strand Ref A C G T 0 24 13 0 1 0 2 0 0 0 24 13 0 3 0 0 0 1 0 24 13 1 0 1 0 0 0 0 24 13 1 3 0 0 0 1 0 24 14 0 0 152 1 4 0 0 24 14 0 1 0 128 0 0 0 24 14 0 2 11 2 196 1 0 24 14 0 3 1 0 2 12 0 24 14 1 0 20 1 2 2 0 24 14 1 1 1 200 1 16 0 24 14 1 2 0 5 118 6 0 24 14 1 3 0 6 3 164 0 24 15 0 0 186 1 2 0 0 24 15 0 1 4 91 0 0 0 24 15 0 2 8 0 90 0 0 24 15 0 3 0 1 2 7 0 24 15 1 0 14 1 0 0 0 24 15 1 1 0 93 1 2 0 24 15 1 2 0 2 79 2